Part:BBa_K743000:Design
Synechocystis PCC6803 neutral recombination site.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
Source
PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.
References
http://genome.kazusa.or.jp/cyanobase/Synechocystis
Kufryk, G. I., Sachet, M., Schmetterer, G., & Vermaas, W. F. J. (2002). Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS microbiology letters, 206(2), 215-9.
Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., & Bryant, D. A. (2011). Photosynthesis Research Protocols. (R. Carpentier, Ed.)Methods, 684, 273-293.
Kucho, K.-ichi, Aoki, K., Itoh, S., & Ishiura, M. (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes & genetic systems, 80(1), 19-23.